Appendix III Efficacy Test Protocols

Implementing Biological Control Agents in the ASEAN Region

Two efficacy test protocols are attached here that are based on a template developed by FAO and were discussed and modified by the Regional BCA Expert Working Group on Application. Both protocols are identical regarding most of the text, but contain specific changes here and there, so that both are included here. The designation as ‘draft’ indicates that the documents may serve as templates for possible Both protocols were distributed to regulatory and application experts of AMS; they originally included as attachment, some notes on the safety, effectiveness, and practical application of selected BCA. That text is not reproduced here, because it encompasses published information, the most important sources of which are referenced below. Microbials DRAFT EFFICACY TEST PROTOCOL This test protocol is concerned with the efficacy evaluation of microbial pest control agents for the control of (common name/scientific name of insect-pest/plant pathogen) in (common name/scientific name of crop). The selection of crop, cultivar and test insects/plant pathogen must be relevant to the (proposed) label/leaflet claims. (Specify objective of the trial and basic information on the trial site like scientific name of the pest and crop, type of trial, environment of trial like field, glasshouse, etc. and any other relevant information) Trials should be conducted only on crops with a known history of uniform high infestation/infection of the target insect-pest(s)/disease(s) (usage of chemical pesticides). Cultural conditions (e.g. soil type and pH, fertilisers, tillage, row and plant spacing, etc.) should be uniform for all the plots of the trial and should conform to local agricultural practices. A series of trials for the relevant pest or disease should be carried out in different locations with distinct environmental conditions over an entire growing period of the crop (e.g. about 2 trials in 2 locations or seasons). The timing, amount and method of irrigation, if applied, should be recorded. Trials can be done under semi-field conditions (e.g. outdoor, but in protected environment or cages) or involving larger scales in farmers’ fields. Generally, highly mobile pests require larger scales than less mobile pests. The relevant conditions of the plot and crop should be adequately described like sowing or planting date, 67 Appendix III Efficacy Test Protocols future versions that would be updated once more application experience accumulates in AMS. 1. EXPERIMENTAL CONDITIONS 1.1 Selection of Crop and Cultivar, Test Organisms 1.2 Trial Conditions row spacing, cultivation measures, crop condition and pest/diseases densities etc.


Implementing Biological Control Agents in the ASEAN Region
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