Test product(s), and untreated control are to be arranged in a randomised block design or any other statistically suitable design. (Describe design and layout of the plots like type of experimental design, number, size and shape of plots and any additional remarks) In the case of on-farm trials, it is recommended to include a negative control, farmers’ practice and the Net plot size: Use an optimum plot size (e.g. 15-20 m2); however, this will depend on the type of crop/pest and disease/product under study and location of trial. Highly mobile pests might require larger plot sizes for evaluation (e.g. 60-80 m2 or larger). Depending on type of the plants/cultivar used; mobility of the test organism, technique of application, type of formulation or application equipment; it may be necessary to take a larger plot size than net plot size or guard or buffer rows/strips are needed to take into account pest dispersal and possible drift of pesticides. Replications: should be 4 per treatment (provided the error or residual degrees of freedom are at least 12). More replications are recommended, in particular, if one wants to account for an expected higher variability of the negative control plots which might show higher pest/disease pressures and crop damage. The method of application (e.g. spray, broadcast, soil application, etc.) will normally be specified on the Different microbial products show different modes of action and require different environmental conditions. Accordingly, there exist specific recommendations for application. A selection of examples is listed in the Annex of this protocol. The application equipment used should be a type in current use, properly calibrated to give intended application rate and droplet spectrum in case of sprays. It should provide an even distribution of product on the whole plot or accurate directional application where appropriate. Factors which may affect efficacy (such 68 1.3 Design and Layout of the Trial 1.3.1 Treatments microbial product under question. 1.3.2 Plot Size and Replication For perennial trees: Net plot size: 2 trees/plot for big trees and 4 trees/plot for small trees. 2. APPLICATION OF TREATMENTS 2.1 Test Product(s) The product(s) under investigation should be the named formulated product(s). 2.2 Mode of Application All applications should comply with good experimental practices. 2.2.1 Method of Application (proposed) label/leaflet. 2.2.2 Type of Equipment Used
Implementing Biological Control Agents in the ASEAN Region
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